In-Vitro Analysis of the Anti-Diabetic Potential of Acetone and n-Hexane Extracts of Delonix regia (Gul Mohar) Leaves

Authors

  • Saeed Ahmed Sheikh Assistant Professor, Department of Pharmacology, Fazaia Ruth Pfau Medical College, Air University, Karachi
  • Asif Ahmed Professor and Head of Department of Pharmacology, Baqai Medical College (BMU), Karachi, Pakistan
  • Shahzad Rasheed Anatomy department college of medicine Imam Mohammad Saud University, Riyadh, KSA
  • S H Waqar Professor of Surgery, Department of General Surgery, PIMS Islamabad
  • Asadullah Lecturer Department of Pharmacology, Shaheed Mohtarma Benazir Bhutto Medical College, Karachi
  • Sama ul Haque Associate Professor Department of Anatomy, Fazaia Ruth Pfau Medical College, Air University
  • Shazia Nawaz Senior Lecturer Department of Pharmacology, Fazaia Ruth Pfau Medical College, Air University, Karachi-74350, Pakistan

DOI:

https://doi.org/10.48036/apims.v21i1.1481

Keywords:

Delonix regia, α-amylase, α-glucosidase, acetone, anti-hyperglycemia

Abstract

Objective: To assess the anti-diabetic activity of leaves extract of D. regia in acetone and n-hexane through in vitro analysis.

Methodology: The preclinical study was conducted in the Department of Pharmacology and Therapeutics at Baqai Medical College/University of Karachi,

in November 2022. Freshly harvested D. regia leaves were collected identified, and authenticated by the herbarium of the botany department at Karachi University. The extract was concentrated in rotary vacuum evaporator and kept in a desiccator to maintain their integrity and suitability for further applications. In vitro, anti-hyperglycemic studies were conducted using ?-amylase and ?-glucosidase inhibition assays.

Results: D. regia leaves extract revealed distinct inhibitory activity of ?-amylase and ?-glucosidase. Acetone extract (25 mg/ml) showed 7.8% and 2.5% inhibition, respectively; while the n-hexane extract (25 mg/ml) demonstrated lower activities, 1.4% and 2.0%, respectively.

Conclusion: The present study concludes that polar solvent (acetone) extract of D. regia exhibits more inhibitory potential for ?-amylase and ?-glucosidase as compares to non-polar solvent emphasizing solvent polarity's role in extracting active compounds.

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Published

2025-01-15

Issue

Vol. 21 No. 1 (2025): January-March

Section

Original Articles

License

Copyright (c) 2025 Saeed Ahmed Sheikh, Asif Ahmed, Shahzad Rasheed, S H Waqar, Asadullah, Sama ul Haque, Shazia Nawaz

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by-nc/4.0/.) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.