Assessment of the Anti-hyperglycemic effects of Rosemary (Salvia Rosmarinus) compared to Glimepiride in Alloxan induced Diabetic Rabbits

Abstract

Objectives:  To observe the antihyperglycemic effects effect of Rosemary (Salvia rosmarinus) in alloxan induced diabetic rabbits.

Methods: The experimental study was conducted at the Department of Pharmacology and Therapeutics in collaboration with the Animal House of Agriculture University, Tando Jam, involved 24 adult healthy male rabbits weighing between 1.5 to 2.5 kg. The rabbits were divided into four groups: Group A (normal control) and Group B (diabetic induced control) both received 0.9% NaCl, while Group C (diabetic induced) received Rosmarinus officinalis 250 mg/kg, and Group D (diabetic induced) received glimepiride 2.5 mg/kg. Clinical parameters such as blood glucose, and body weight were measured on day 0 and day 28. Rosmarinus officinalis leaves were dried, powdered, and prepared into an ethanolic extract. Blood samples were collected from the femoral vein, centrifuged, and analyzed for serum blood glucose level. Hyperglycemia was confirmed with blood glucose levels above 200 mg/dl. Data was entered and analyzed by SPSS version 26.

Results: Fasting blood sugar (FBS) levels showed a significant reduction in Groups C and D compared to Group B by Day 28. Specifically, in Group C' FBS dropped from 252.8±2.5 to 181.3±2.3, and in Group D from 255±1.8 to 197.16±2.5, indicationg Rosmarinus officinalis demonstrated significant efficacy in improving FBS levels in diabetic rabbits.

Conclusion: Rosmarinus officinalis effectively reduced glycemic concentrations in alloxan-induced diabetic rabbits..