A Study of Antimicrobial Effects of Citrus Paradisi ‘Grape Fruit’
Objectives: In the current study, antimicrobial action of citrus paradisi grape fruit against common pathogens was evaluated. Staphylococcus aureus is a gram positive coccus, forming grape-like clusters on agar culture.
Methodology: Extracts were prepared with two different concentrations: 25 mg extract/1ml DMSO and 50 mg extract/1ml DMSO. The antibacterial activities were determined by using different concentrations of the prepared extracts. Mueller Hinton agar plates were carefully inoculated with 10 µl of bacterial spread, and plates were then properly labeled. The discs were placed on agar plates by means of a plain forceps. 20 µl of prepared extracts with concentration of 50 mg extract/1ml DMSO were added on the discs of half of the plates, while 10 µl of each extract was added on the discs of remaining plates. These plates were then incubated at 37oC for one day. Same procedure was repeated with 25 mg extract/1ml DMSO.
Results: The extracts utilized against Bacillus Cereus were labeled as CF-04, CF-12, CF-13 and CF-14. Inhibition in CF-14 was observed to be highest, compared with other sections, showing the activity of ciprofloxacin to be effective in this culture.
Discussion: Current study evaluated the antioxidant and antimicrobial potential of extracts of different parts of citrus paradisi plant prepared in ethyl acetate solution. This study demonstrated the antimicrobial effects of parts of grape fruit plant against six common bacterial strains and highly positive zone of inhibitions were observed.
Conclusion: In all these prepared extracts, CF-10 showed maximum zone of inhibition. The prepared extracts utilized against K pneumoniae were CF-02, CF-04, CF-10 and CF-14, which were prepared from pulp, peel, leaves and stem of citrus paradisi respectively. In all these prepared extracts, CF-14 showed maximum zone of inhibition.