Prevalence of Dermatophytes and their Antifungal Susceptibility to itraconazole in Clinically Suspected Patients of TInea Corporis
Abstract
Objective: To assess the prevalence and clinico-mycological characteristics of dermatophyte species in tinea corporis, and determine the susceptibility of isolated dermatophyte species to Itraconazole.
Methods: Current cross sectional study was conducted in Department of Microbiology, Basic Medical Sciences Institute, Karachi. A total of 301 samples of skin scraping of the suspected cases of tinea corporis were taken from the patients who visited dermatology OPD of Jinnah Postgraduate Medical Centre. The specimens were taken by scrapping the margin of lesions with a sterile surgical blade and were processed for potassium hydroxide (KOH) mount test. The mycological culture was performed on Sabouraud’s dextrose agar with and without antibiotics and on dermatophyte test medium. Lactophenol cotton blue mount, cellophane tape mount, and biochemical testing were used to identify the species of dermatophytes. The antifungal susceptibility testing was done by micro broth dilution method according to the guideline of Clinical and Laboratory Standard Institute. The storage and analysis of data was perfomed on Statistical Package for Social Sciences Version 21.
Results: The males were affected more than females. Redness/ Erythema was the most common presenting complaint (n= 192, 63.8%) while the most common clinical type was an annular lesion (n= 133, 44.2%). The most common isolated dermatophytes was T. mentagrophytes (n= 38, 45.2.%). There were no resistance seen towards itraconazole for any isolates, though the MICs of itraconazole was within the range in the upper limit for almost every isolate. This indicates the need to optimize the use of itraconazole. Furthermore, because itraconazole is the last medicine in the existing arsenal, so itraconazole must be used wisely
Conclusion: Trichophyton formed the commonest etiological agent and T. mentagrophytes and T. rubrum were found to be the principal causative agent of tinea corporis in our region. Isolates were found to be susceptible to itraconazole, however the MICs were found to be in the upper range.
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Copyright (c) 2024 Vinita Kumari, Suresh kumar, Yalpa Kumari, bhawani shanker, Mohammed Salman Zafar, Syed Sohail Abbas
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